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. 2026 Mar 30;11(4):e00084-26. doi: 10.1128/msphere.00084-26

Fig 2.

Transcriptome analysis of nri mutants showing gene expression changes through volcano plots, overlap of altered genes between nri1Δ/Δ and nri1Δ/Δ nri2Δ/Δ via Venn diagrams, and affected biological processes from gene ontology analysis.

nri mutants exhibit global alterations in the transcriptome profile. (A) Volcano plot representations of the differentially expressed genes (DEGs) for nri1Δ/Δ w.r.t. WT (nri1Δ/Δ) and nri1Δ/Δ nri2Δ/Δ w.r.t. WT (nri1Δ/Δ nri2Δ/Δ) samples. Significantly altered genes with the threshold of −1<log2(Fold Change)>1 and FDR (adjusted P-value) <0.05 are highlighted in blue and red colors, respectively. (B) Venn diagram indicating overlap between the upregulated and downregulated genes in nri1Δ/Δ and nri1Δ/Δ nri2Δ/Δ mutants. Numbers in the parentheses denote the total number of up-regulated and down-regulated DEGs in the indicated mutant strains. (C) Gene ontology (GO) analysis of DEGs in nri1Δ/Δ and nri1Δ/Δ nri2Δ/Δ samples. The Y-axis indicates various GO terms of the biological processes, and the X-axis indicates percentage of the DEGs encompassing that GO term.