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. 2026 Mar 26;11(4):e00884-25. doi: 10.1128/msphere.00884-25

Fig 2.

Fluorescence microscopy tracking PfGEX1-GFP expression in parasites. No signal in asexual stages or early gametocytes. GFP localizes to the nuclear envelope in stage III–V gametocytes from days 6 to 11 of development.

PfGEX1 marks the nuclear envelope in late-stage gametocytes. (A) Top: schematic illustration of the modified pfgex1 locus in NF54/GEX1-GFP parasites. Bottom: representative live cell fluorescence images of ring stage (R), trophozoites (T), and schizonts (S) showing lack of PfGEX1-GFP expression during the asexual intra-erythrocytic development cycle (IDC). (B) Representative live cell fluorescence images of gametocytes throughout sexual development (days 2–11) showing PfGEX1-GFP expression in stage III–V gametocytes (days 6–11). DNA was stained with Hoechst. DIC, differential interference contrast. Scale bar, 5 μm.