Table 2. Expression of MMP-9, MMP-2, and TIMP-2 in tumor and healthy tissue.

MMP-9 immunohistochemistry
Normal tissue intensity staining
Nuclear epithelium		Cytoplasmic epithelium		Inflammatory cells		Stromal cells		Endothelial cells
0+	3	0+	0	0+	0	0+	0	0+	0
1 +	15	1+	6	1+	0	1+	0	1+	0
2+	3	2+	15	2+	8	2+	12	2+	10
3+	0	3+	0	3+	14	3+	10	3+	12
Proportion of staining normal tissue (%)
Nuclear Staining		Cytoplasmic Staining		Inflammatory cells		Stromal cells		Endothelial cells
≤25%	4	≤25%	1	≤25%	0	≤25%	0	≤25%	0
26–50%	11	26–50%	13	26–50%	1	26–50%	0	26–50%	0
51–75%	0	51–75%	0	51–75%	0	51–75%	0	51–75%	0
>75%.	6	>75%.	7	>75%.	21	>75%.	22	>75%.	22
Tumor tissue intensity staining
Nuclear		Cytoplasmic		Inflammatory cells		Stromal cell		Endothelial cells
0+	4	0+	0	0+	0	0+	1	0+	0
1+	31	1+	0	1+	0	1+	0	1+	0
2+	4	2+	18	2+	15	2+	17	2+	18
3+	1	3+	22	3+	25	3+	22	3+	22
Proportion of staining in tumor cells (%)
Nuclear		Cytoplasmic		Inflammatory cells		Stromal cell		Endothelial cells
≤25%	4	≤25%	0	≤25%	0	≤25%	1	≤25%	0
26–50%	1	26–50%	0	26–50%	1	26–50%	0	26–50%	0
51–75%	0	51–75%	0	51–75%	0	51–75%	0	51–75%	0
>75%.	35	>75%.	40	>75%.	39	>75%.	39	>75%.	40
MMP-2 immunohistochemistry
Normal tissue intensity staining
Nuclear epithelium		Cytoplasmic Epithelium		Inflammatory Cells		Stromal Cells		Endothelial Cells
0+	2	0+	0	0+	1	0+	0	0+	0
1+	6	1+	7	1+	1	1+	1	1+	2
2+	12	2+	15	2+	13	2+	13	2+	13
3+	3	3+	1	3+	6	3+	7	3+	6
Proportion of staining normal tissue (%)
Nuclear Staining		Cytoplasmic Staining		Inflammatory Cells		Stromal Cells		Endothelial Cells
≤25%	2	≤25%	2	≤25%	1	≤25%	0	≤25%	0
26–50%	7	26–50%	12	26–50%	0	26–50%	0	26–50%	0
51–75%	6	51–75%	1	51–75%	0	51–75%	0	51–75%	0
>75%.	8	>75%.	8	>75%.	20	>75%.	21	>75%.	21
Tumor tissue intensity staining
Nuclear		Cytoplasmic		Inflammatory Cells		Stromal Cells		Endothelial Cells
0+	0	0+	0	0+	0	0+	0	0+	1
1+	6	1+	0	1+	0	1+	0	1+	0
2+	29	2+	10	2+	23	2+	23	2+	20
3+	4	3+	29	3+	14	3+	15	3+	11
Proportion of staining in tumor cells (%)
Nuclear		Cytoplasmic		Inflammatory Cells		Stromal Cells		Endothelial Cells
≤25%	0	≤25%	0	≤25%	0	≤25%	0	≤25%	1
26–50%	0	26–50%	0	26–50%	1	26–50%	0	26–50%	0
51–75%	0	51–75%	0	51–75%	0	51–75%	0	51–75%	0
>75%.	39	>75%.	39	>75%.	36	>75%.	38	>75%.	31
TIMP-2 immunohistochemistry
Normal tissue intensity staining
Nuclear epithelium		Cytoplasmic Epithelium		Inflammatory Cells		Stromal Cells		Endothelial Cells
0+	2	0+	0	0+	1	0+	0	0+	0
1 +	6	1+	7	1+	1	1+	1	1+	2
2+	12	2+	15	2+	13	2+	13	2+	13
3+	3	3+	1	3+	6	3+	7	3+	6
Proportion of staining normal tissue (%)
Nuclear Staining		Cytoplasmic Staining		Inflammatory Cells		Stromal Cells		Endothelial Cells
≤25%	0	≤25%	2	≤25%	1	≤25%	0	≤25%	0
26–50%	7	26–50%	12	26–50%	0	26–50%	0	26–50%	0
51–75%	6	51–75%	1	51–75%	0	51–75%	0	51–75%	0
>75%.	8	>75%.	8	>75%.	20	>75%.	21	>75%.	21
Tumor tissue intensity staining
Nuclear		Cytoplasmic		Inflammatory Cells		Stromal Cells		Endothelial Cells
0+	0	0+	0	0+	0	0+	0	0+	1
1+	6	1+	0	1+	0	1+	0	1+	0
2+	29	2+	10	2+	23	2+	23	2+	20
3+	4	3+	29	3+	14	3+	15	3+	11
Proportion of staining in tumor cells (%)
Nuclear		Cytoplasmic		Inflammatory Cells		Stromal Cells		Endothelial Cells
≤25%	0	≤25%	0	≤25%	0	≤25%	0	≤25%	1
26–50%	0	26–50%	0	26–50%	1	26–50%	0	26–50%	0
51–75%	0	51–75%	0	51–75%	0	51–75%	0	51–75%	0
>75%.	39	>75%.	39	>75%.	36	>75%.	23	>75%.	31