Fig. 1.

Increase in the number of Olig2-immunoreactive cells after a stab wound. (A and B) Frontal sections of the gray matter (GM) in the adult mouse neocortex stained for Olig2. Note the increase in the number of Olig2+ cells after a stab wound (s-w, B; yellow line indicates the lesion site, dashed contours outline some unspecific/autofluorescent staining, and arrowheads point to some Olig2+ cells) compared to the intact cortex (A, arrowheads indicate Olig2+ cells) as quantified in the histogram (C). *, statistically significant differences; hs, hours; dpl, days after lesion. In situ hybridization reveals Olig2 mRNA up-regulation after a stab wound (E) compared to the intact cortex (D). (Scale bars: 100 μm.)