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. 2005 Dec 5;102(50):18183–18188. doi: 10.1073/pnas.0506535102

Fig. 2.

Fig. 2.

Identification of Olig2-immunoreactive cells after stab-wound and proliferation analysis. (A–E) Frontal sections of the intact neocortical GM immunostained as indicated. Olig2 immunoractivity was detected in CC1+ oligodendrocytes (A), NG2+ glial precursors (B), and S100β+ astrocytes (C). Arrows point to colabeled cells, and arrowheads point to cells devoid of Olig2. (D) Olig2 and Sox10 coexpression (arrows) in the cortical GM are shown. (E) The micrograph depicts Olig2+ nuclei triple labeled for BrdUrd (red) and a mixture of antibodies (in blue: NG2, CC1, and S100β) close to the stab-wound track: Several proliferating Brdu+/Olig2+ cells are lineage marker-negative (arrow). Histograms in F depict the proportions of cell types in the Olig2+ population in the intact cortex and after a stab wound, whereas in G, the percentage of Olig2+ cells amongst different glial populations is shown. The fractions of Olig2+ astrocytes increased at 7 dpl (P = 0.012) compared to the intact situation. The opposite was observed for the NG2+ cells in comparison with the intact cortex (P = 0.003 at 3 days; P = 0.042 at 7 days) and the proportion of Olig2+/CC1+ oligodendrocytes rose 7 dpl (P = 0.044). (H) Olig2/BrdUrd colabeled cells (indicated also as percentages) are shown over the density of BrdUrd-positive cells in the intact and stab-lesioned cortex (2-h BrdUrd pulse). (I) The composition of BrdUrd-positive cells is depicted after 2 h (Left) or several days of BrdUrd pulse (Right). (Scale bars: A–C and E, 20 μm; D, 100 μm.)