Repression of Olig2 function after a stab wound. (A) The viral injection experimental design is illustrated: Animals were injected with either the GFP or the Olig2VP16 virus 2 days after a stab wound and killed later. The micrograph (B) illustrates the distribution of GFP+-infected cells in the cortex 7 days after control virus injection (GM, gray matter; WM, white matter). (C–I) GFP+ cells 7 days after viral transduction (as specified in the images) stained for the antigens indicated. (F) Arrow points to a marker-negative infected cell. The micrographs (I–I′′′) show examples of Pax6 and dcx coexpression in Olig2VP16-infected cells. The graphs in J–L illustrate the phenotypes of virus-infected cells as indicated (three mice analyzed for cell type identification at each time point; 100–200 analyzed cells unless differently indicated in the text; dpi, days after injection). (Scale bars: B, 200 μm; C–G,20 μm; H,40 μm; I–I′′′,10 μm.)