Fig. 6.

The cytosolic inhibition of heat-induced cytochrome c release is reversed by BH3-only protein or peptide. (A) Mouse-liver mitochondria were enriched and combined with 5 mg/ml Jurkat CE and, where indicated, heated at 43°C for 60 min before the addition of increasing amounts of active N/C Bid. (B) Enriched mitochondria were heated as in A and incubated with 10 μM Puma peptide after heat treatment. All samples were incubated at 37°C for 1 h, and mitochondria (pellet) and supernatant were assessed for cytochrome c release by Western blot. The pellet fraction was also probed for Hsp60 to confirm equal loading of the samples.