Fig. 2.

The decoy receptor TR4 is a low-affinity receptor for TRAIL but effectively inhibits apoptosis signaling by TR2 in Jurkat cells. (A) Jurkat 4E3 cells were treated with TRAIL in the presence of the indicated amount of soluble TRAIL receptor Fc fusion proteins. Cell death was determined by flow cytometry. (B) The indicated plasmids were transfected into Jurkat 4E3 cells and stimulated with the indicated doses of TRAIL or anti-Fas antibody. Apoptosis was monitored by Annexin V staining. (C) Jurkat 4E3 cells were transfected with the indicated plasmids, stimulated with TRAIL, and analyzed for cell death. Results are representative of three independent experiments.