Skip to main content
. 2005 Dec 5;102(50):18135–18140. doi: 10.1073/pnas.0507798102

Fig. 1.

Fig. 1.

Generation of Tg-rPF4/APP transgenic mice. (a) Schematic of the 4.3-kb transgene construct used to generate the Tg-rPF4/APP transgenic mice containing the rat platelet factor-4 promoter, a cDNA of human APP770, and a poly(A) tail from bovine growth hormone. (b) PCR analysis was performed for human AβPP transgene in DNA isolated from Tg-rPF4/APP mice (lane 2) and wild-type mice (lane 3) as described in Methods. Lane 1 presents a 1-kb ladder. (c) Immunoblot analysis for human PN2/AβPP expression in platelet lysates from wild-type (lane 1), heterozygous Tg-rPF4/APP (lane 2), and homozygous Tg-rPF4/APP (lane 3). The mAb P2-1, which is specific for human AβPP, was used.