Skip to main content
. 2005 Dec 5;102(50):18135–18140. doi: 10.1073/pnas.0507798102

Fig. 3.

Fig. 3.

Transgene-encoded human PN2/AβPP is released from Tg-rPF4/APP platelets upon activation and affects clotting in vitro. (a) Platelets isolated from Tg-rPF4 mice were incubated in the absence or presence of 20 μg/ml collagen or 10 nM thrombin. The platelet pellets (P) (lanes 1, 3, and 5) and platelet releasates (R) (lanes 2, 4, and 6) were analyzed for human PN2/AβPP by immunoblotting with mAb P2-1 as described in Methods. (b) Gel-filtered platelets were isolated from PRP prepared from wild-type, Tg-rPF4/APP, and AβPP KO mice and analyzed for aggregation after stimulation with collagen or thrombin as described in Methods. (Scale bar, 4 min.) (c) PRP was prepared from freshly collected blood from wild-type, Tg-rPF4/APP, and AβPP KO mice and then analyzed for in vitro clotting by using an APTT assay as described in Methods. Data shown are mean ± SD of n = 6 mice per genotype. *, P < 0.001.