PN2/AβPP affects thrombosis in vivo.(a) Mice of different genotypes were injected with the photoactivated dye rose bengal at t = 0, and the carotid artery was exposed to a laser light. Blood flow through the carotid artery was monitored with a flow probe. Blood flow ceased because of thrombus formation in the vessel. Representative experiments show wild-type mice (solid line), Tg-rPF4 mice (dotted line), and AβPP KO mice (dashed line). (b) Quantitation of the time to cessation of carotid artery blood flow in mice of different genotypes. Data shown are the mean ± SD from Tg-rPF4/APP mice (n = 11), wild-type (n = 30), and AβPP KO mice (n = 13). *, P < 0.0001.