Fig. 2.

H₂O₂ induced necrosis and apoptosis in wild-type (blue bars) and mtDNA mutator (red bars) primary MEF cultures. Cells were stained with propidium iodide and annexin V and assayed for viability (mean values ± SD) by FACS analysis after 1 h incubation in culture media containing 0.5 mM (A), 2 mM (B), and 10 mM (C) H₂O₂ or in medium without H₂O₂ (D). Asterisks indicate level of statistical significance: *, P < 0.05; **, P < 0.01.