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Expression of antioxidant defense enzymes. (A) Northern blot analyses showing transcript levels of GPX1 and SOD2 in hearts of 25- and 40-week-old mice. The levels of 18S ribosomal RNA was used as loading control. (B) Western blot analysis showing SOD2 protein levels in total protein extracts from heart, liver, and purified liver mitochondria at 40 weeks of age. Mut, mutants; wt, wild types.
