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. 2005 Dec 6;102(50):18034–18039. doi: 10.1073/pnas.0509109102

Fig. 2.

Fig. 2.

TAZ and YAP play important roles in cardiac ANF expression. (A) Structure of mouse TAZ and YAP proteins. WW, WW1, and WW2, WW domain. TAZ and YAP share WW domain(s), a coiled-coil motif, and a PDZ-binding motif. YAP contains a proline-rich domain and an SH3-binding motif. (B) YAP activates TBX5-dependent ANF-luciferase expression in a dose-dependent manner. TBX5, 10 ng; YAP, 5–200 ng. Basal luciferase activity without TBX5 and YAP expression was given a value of 1. (C) siRNA against TAZ or YAP reduced ANF-luciferase activity in cardiac myocytes. Primary neonatal mouse cardiac myocytes were cotransfected with siRNA and ANF-luciferase plasmid. siRNA, control (siRNA without known homology with mammalian sequences) (Qiagen); TAZ-site #1 and -site #2, against mouse TAZ; YAP-site #1 and -site #2, against mouse YAP. Luciferase activity with control siRNA cotransfection was given a value of 100%.