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. 2005 Dec 6;102(50):18034–18039. doi: 10.1073/pnas.0509109102

Fig. 5.

Fig. 5.

Effects of TAZ on various TBX5 mutants identified in HOS patients. (A) Premature-termination mutants identified in HOS patients, TBX5-R279Ter and -E316Ter, show marked reduction of TAZ-mediated transactivation. (Left) Structures of TBX5 mutants are shown. Numbers indicate the positions of the T-box and amino-/carboxyl-termini of full-length protein in the amino acid sequence. Asterisks indicate the positions of point mutations. COS-1 cells were cotransfected with expression plasmids encoding TBX5 or HOS mutants (10 ng) and/or TAZ plasmid (100 ng). Expression of TBX5 proteins and TAZ was confirmed by Western blotting. Lane numbers on the gel correspond to transfections (Right). (B) DNA-binding activity of TBX5-R279Ter and -E316Ter mutants is not affected. Electrophoretic mobility shift assay is shown. Arrowheads indicate shifted bands. (C) TBX5-R279Ter and -E316Ter show inefficient interaction with TAZ. Coimmunoprecipitation was performed as described in Fig. 3.