Table 2.

Polymerase chain reaction protocol for lactic acid bacteria (30 cycles) and yeasts (35 cycles)

Primers	Initial denaturationw	30/35 cycles	Final extension	Initial elongation	Final elongation
		Denaturation	Hybridization
Lb-F Lb-R	95°C for 3 min	94°C for 30 s	50°C for 30 s	72°C for 90 s	72°C for 5 min
ITS4-F ITS5-R	95°C for 10 min	94°C for 30 s	55°C for 30 s	72°C for 1 min	72°C for 7 min