Table 4. Biochemical and spectroscopic characterization of FDP-ΔRd and mutants.
The g values resulted from EPR spectral simulations.
| Cofactor analysis | Enzymatic activity | ||||
|---|---|---|---|---|---|
| Protein | Iron/protein | Flavin/protein | O2 reduction (s−1) | NO reduction (s−1) | EPR (g values of the mixed-valence sate) |
| FDP-ΔRd | 1.15 ± 0.09 | 0.71 ± 0.03 | 0.68 ± 0.07 | 0.46 ± 0.05 | 1.94, 1.80, 1.74 |
| D52K | 1.20 ± 0.20 | 0.59 ± 0.06 | 0.22 ± 0.04 | 0.21 ± 0.09 | 1.95, 1.78, 1.72 |
| S262Y | 1.03 ± 0.18 | 0.64 ± 0.03 | 0.39 ± 0.07 | 0.42 ± 0.11 | 1.95, 1.80, 1.74 |
| D52K/S262Y | 1.24 ± 0.21 | 0.75 ± 0.08 | 0.60 ± 0.12 | 0.25 ± 0.06 | 1.96, 1.78, 1.74 |