Fig. 4.

Zymosan-modulated neutrophils exhibit an alternatively activated phenotype. A Schematic illustrating the generation and isolation of zymosan-modulated neutrophils (ZMN) from mice following intraperitoneal administration of zymosan. B, C ZMN, isolated from i.p. zymosan injected from Arg1^YFP reporter mice, and bone marrow neutrophils (BMN) isolated from naïve from Arg1^YFP reporter mice, were analyzed by flow cytometry. B t-distributed stochastic neighbor embedding (t-SNE) analysis of ZMN (blue) and BMN (green), gated as viable CD45⁺CD11b⁺Ly6G⁺ cells. C t-SNE feature plots (left) and corresponding histograms (right) depicting expression of the indicated markers across the neutrophil clusters. Gray histograms represent controls used to define gating thresholds: ZMN from a non-reporter mouse for Arg1/YFP and fluorescence minus one (FMO) controls for the remaining markers. Neutrophils were pooled from three mice per group in a single experiment, representative of two independent experiments