Fig. 7.

Zymosan-modulated neutrophil–mediated immunosuppression is independent of PD-L1. A Representative histograms showing PD-L1 expression on naive bone marrow–derived neutrophils (BMN; green) and zymosan-modulated neutrophils (ZMN; blue), with fluorescence-minus-one (FMO) control shown as a gray dotted line. Neutrophils were gated as viable CD45⁺CD11b⁺Ly6G⁺ cells. B Representative CFSE dilution plots of naïve CD4⁺ T cells stimulated with anti-CD3 and anti-CD28 and cultured either alone or in co-culture with ZMN in the presence or absence of PD-L1–blocking antibodies. Quantification of the percentage of CFSE.^low dividing cells among total CD4⁺ T cells (gated on viable CD45⁺CD11b⁻CD4⁺ cells) is shown in the bottom panel. Data represent 2–3 biological replicates per group pooled from two independent experiments, with each biological replicate comprising 2–3 technical replicates. Statistical significance was determined using a two-tailed Student’s t test. Data are presented as mean ± SEM. p < 0.05