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Dot blot hybridization detection of multiple HPV types after TDP3 PCR. HPV types 16, 45, 51, and 52 were combined (1.5pg, 150fg, 15fg, 1.5fg, or 150ag of each type) in a background of 100 ng C-33A DNA. After PCR, 0.6 μl of denatured product was spotted onto nylon membrane and hybridized with biotin-labeled HPV type specific oligonucleotide probe. Duplicate dot preparations were prepared.
