Table 2.

PCR protocol sensitivities for the detection of HPV-16 in SiHa/C-33A DNA mixtures. SiHa cell DNA (0.1 ng to 100 ng) was mixed with C-33A cell DNA to give final DNA quantities of 2 μg, 1 μg, 0.5 μg, or 0.1 μg. These preparations were tested with up to six different PCR protocols. HPV-16 detection was most successful using by the TDP3 and TDP4 conditions. Data are for protocols tested at 50 PCR cycles. HPV detected, HPV undetected, ND: not done.

	Total DNA in PCR: 2 μg					Total DNA in PCR: 1 μg
SiHa DNA	100 ng	25 ng	1 ng	0.5 ng	0.1 ng	100 ng	25 ng	1 ng	0.5 ng	0.1 ng
GP5+/6+
TDP1
TDP2
TDP3
TDP4	ND	ND	ND	ND	ND
MRT	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
	Total DNA in PCR: 0.5 μg					Total DNA in PCR: 0.1 μg
SiHa DNA	100 ng	25 ng	1 ng	0.5 ng	0.1 ng	100 ng	25 ng	1 ng	0.5 ng	0.1 ng
GP5+/6+
TDP1
TDP2
TDP3
TDP4
MRT	ND	ND	ND	ND	ND