Table 7.
Confirmation of gene array expression by real time RT-PCR for a select list of genes.a
| 2 ppm
|
5 ppm
|
||||
|---|---|---|---|---|---|
| Gene symbolb | Gene nameb | Gene array | RT-PCR | Gene array | RT-PCR |
| c-erb | thyroid hormone receptor | 5.0c | 3.0 | NC | NC |
| c-jun | transcription factor AP1 | NC | NC | 5.0 | 3.0 |
| Nos2 | inducible nitric oxide synthase | NC | NC | 2.0 | 1.8 |
| Gsr | glutathione reductase | 5.0 | 5.2 | NC | NC |
| Igfbp2 | insulin-like growth factor binding protein 2 | − < 15 | −20.0 | − < 5.0 | −5.5 |
NC, no change in expression.
a
Log numbers derived from real-time PCR analysis were normalized to the expression of the housekeeping gene GAPDH , which was unaltered by O3 exposure in rat lung tissue.
b
Gene symbols and names derived from the Duke Integrated Genomics Database (https://dig.cgt.duke.edu/try_query.php).
c
Fold change in expression compared with air-exposed control rat lung tissue.