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. 2005 Dec 16;1(6):e77. doi: 10.1371/journal.pgen.0010077

Figure 4. DHC Increases p53 Acetylation, Cytotoxicity, and Apoptosis in the TK6 Cell Line.

Figure 4

(A) DHC increases p53 lysine 373 and 382 acetylation in a dose-dependent manner in the TK6 cell line following a 24-h exposure period. The immunoblot is representative of three separate experiments.

(B) DHC increases cytotoxicity in a dose-dependent manner following a 24-h exposure to DHC. The average ± standard error for each of three experiments is shown.

(C) A 5-mM dose of DHC increases apoptosis at the 6-h time point in the TK6 cell line. A combination of annexin V fluorescein isothiocyanate and propidium iodide staining was used to discriminate among apoptotic (lower-right box) and necrotic (upper-right box) cells. Figures are representative of three separate experiments.

(D) DHC increases apoptosis in a dose-dependent manner in the TK6 cell line at the 6-h time point. The average ± standard error for three experiments is shown.