Figure 4.

Toll-like Receptor (TLR) mRNA expression by stimulated primary small-airway-epithelial cells (SAEC). SAEC were stimulated for 6 h (black bars) or 24 h (gray bars) with different TLR ligands including LPS, flagellin, poly(I:C), macrophage activating lipopetide-2 (MALP-2) and zymosan. TLR1 – TLR6 mRNA expression was analyzed by quantitative RT-PCR. Results were normalized using β-actin as endogenous control and are shown as fold changes relative to untreated controls. TLR6 protein expression in unstimulated (U) and poly(I:C) stimulated (I:C) SAEC was analyzed by Western blotting and is shown as an insert in the diagram.