Figure 9.

mRNA expression of genes involved in TLR signaling in stimulated primary small-airway-epithelial cells (SAEC). SAEC were stimulated for 6 h (black bars) or 24 h (gray bars) with different cytokines. MyD88, TRIF, IRAK-2 and IRAK-3 mRNA expression was analyzed by quantitative RT-PCR. Results were normalized using GAPDH as endogenous control and are shown as fold changes relative to untreated controls. No changes of the gene expression of TRAM, TIRAP, TOLLIP, IRAK-1 and IRAK-4 were observed in the cytokine stimulated cells (data not shown).