Figure 1.

Map of the ∼3.5-Mb Region Spanning the Recombination-Free Region, Including the Centromere, of Rice Chromosome 8.

The recombination-free region is located at 54.3 cM on the genetic linkage map and spans ∼2312 kb. This region is divided into three domains. Domain A includes the ∼750-kb CENH3 binding region (in pink) (Nagaki et al., 2004). Domains B1 and B2 (in light green) flank domain A. The two distal domains, C1 and C2, flank the recombination-free region. Sequences of 34 BAC/PAC clones (gray bars) were downloaded from GenBank and assembled into the Cen8 virtual contig of 3,489,829 bp. Relative position and overlapping of these clones was determined using National Center for Biotechnology Information (NCBI) Blast2seq. The 10 genetically mapped RFLP markers in this region are shown at the top of the map.