Figure 3.

Sequence Features along the ∼3.5-Mb Cen8 Virtual Contig.

Genes were annotated using a combination of transcript/protein homolog search and ab initio prediction and then tested for expression using RT-PCR. Repetitive DNA was identified using RepeatMasker with the TIGR Oryza repeat database (Ouyang and Buell, 2004) and centromere retrotransposon of rice (Nagaki et al., 2005) as the filters. The cutoff is a minimum score of 225 and ≤35% of divergence. All genes and repeats are plotted against their coordinates on the virtual contig.