Figure 7.

Dual Expression of Arabidopsis PEX4 and PEX22 Rescues Yeast pex4 and pex22 Mutant Defects.

Wild-type, Δpex4, and Δpex22 yeast strains were transformed with the indicated combinations of empty vectors or vectors expressing Arabidopsis (At) PEX4 or PEX22 from the constitutive GPD promoter. Yeast strains were examined microscopically as described in the legend to Figure 4A. Wild-type fluorescence is punctate, indicating peroxisomal localization of GFP, whereas Δpex4 and Δpex22 display diffuse cytoplasmic fluorescence unless Arabidopsis PEX4 and PEX22 cDNAs are coexpressed, which partially restores the punctate pattern. All images were made at ×100 magnification using the same exposure time. Bar = 50 μm.