Figure 8.

ICL Is Stabilized in the pex4-1 pex22-1 Mutant.

Extracts from wild-type and pex4-1 pex22-1 seedlings grown for 4, 6, or 9 d in the light on medium with sucrose were analyzed by immunoblotting using α-ICL (top). Four-day-old icl-1 seedlings, in which the single-copy ICL gene is disrupted by an En-1 insertion in exon 3 (Eastmond et al., 2000), were used as a control for the castor bean ICL antibody (Maeshima et al., 1988). This antibody detects a protein of the expected size of Arabidopsis ICL (66 kD), which is absent in the icl-1 mutant, and a smaller unidentified cross-reacting band (asterisk). The bottom panel shows the membrane reprobed with α-HSC70 as a loading control. The positions of molecular mass markers (kD) are shown at left.