Figure 5.

Enhanced Drought Tolerance in 35S-AREB1ΔQT Plants.

(A) Enhanced tolerance to drought in the 35S-AREB1ΔQT plants (lines 12 and 26). Watering was withheld from 3-week-old plants for 12 d, then rewatering for 10 d, before the photograph was taken. Number codes indicate number of surviving plants out of total number.

(B) Enhanced ability of 35S-AREB1ΔQT plants (line 12) to survive the dehydration condition. Three-week-old transgenic and wild-type plants were grown on GM agar plates, transferred to Petri dishes, left unwatered for 6 h, and then rewatered.

(C) Increased survival rates of the 35S-AREB1ΔQT plants (lines 12 and 26) under dehydration. Water was withheld for 5 to 6 h from 3-week-old plants and then survival rates were counted. Surviving plants were scored on the second day. Survival rates and standard deviations (bars) were calculated from results of three independent experiments.

(D) Water loss rates of 35S-AREB1ΔQT (lines 12 and 26) plants. Each data point represents the mean of duplicate measurements (n = 7 each). Error bars represent standard deviation.

(E) Standardized water content of 35S-AREB1ΔQT (lines 12 and 26) plants. Details as in (D). Some error bars are smaller than the symbols.

(F) Stomatal aperture of 35S-AREB1ΔQT plants (line 12). Stomatal guard cells were observed in the middle of the watering cycle.