Table 1.
Genes Upregulated in Plants Overexpressing AREB1ΔQT, Identified by Microarray Analysis
| Experiment 1 (Transgenic Line 8)a |
Experiment 2 (Transgenic Line 12)a |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Inducibilityb | Genec | Descriptionc | RAFL Clone No.d | AGI Codee | Median of Fold Changef | Fold Change 1 | P Value 1g | Fold Change 2 | P Value 2g | Fold Change 1 | P Value 1g | Fold Change 2 | P Value 2g | No. of ABREsh | |||
| D | – | A | – | HIS1-3 | Linker histone H1 | RAFL05-20-P13 | AT2G18050 | 15.5 | 17.6 | 5.6E-32 | 20.9 | 1.5E-32 | 12.1 | 3.2E-30 | 13.3 | 1.1E-30 | 2 |
| D | S | A | – | AIA1 | AAA family ATPase | RAFL08-19-A04 | AT1G64110 | 13.3 | 12.8 | 9.1E-30 | 8.6 | 7.1E-26 | 13.9 | 1.1E-30 | 16.1 | 1.2E-30 | 2 |
| D | S | A | – | AIL1 | LEA class protein | RAFL15-06-F04 | AT3G17520 | 13.0 | 9.8 | 1.2E-28 | 8.5 | 4.0E-27 | 28.0 | 2.2E-33 | 16.2 | 1.5E-31 | 2 |
| – | – | – | – | Dihydroflavonol 4-reductase | AT5G42800 | 7.9 | 15.1 | 8.2E-30 | 7.5 | 3.2E-24 | 6.4 | 3.6E-22 | 8.3 | 4.9E-21 | 2 | ||
| D | S | A | – | RD29B | Transcription factor | RAFL05-11-I09 | AT5G52300 | 6.6 | 9.8 | 2.0E-27 | 4.6 | 2.1E-18 | 7.3 | 6.5E-25 | 6.0 | 7.2E-21 | 5 |
| D | S | A | – | GBF3 | Transcription factor | RAFL05-09-G15 | AT2G46270 | 5.7 | 5.2 | 1.7E-22 | 4.0 | 1.2E-18 | 6.9 | 6.7E-26 | 6.2 | 2.6E-24 | 8 |
| D | S | A | C | RD20 | Ca2+ binding EF-hand protein | RAFL08-16-M12 | AT2G33380 | 5.5 | 6.5 | 1.3E-25 | 7.3 | 1.6E-26 | 4.5 | 1.9E-21 | 4.5 | 3.1E-21 | 3 |
| D | S | A | C | RAB18 | LEA class protein | RAFL05-18-M18 | AT5G66400 | 5.5 | 5.7 | 3.2E-24 | 4.1 | 5.0E-20 | 6.0 | 8.0E-25 | 5.2 | 4.0E-23 | 5 |
| – | – | A | – | Protease inhibitor/seed storage/LTP family | RAFL05-12-N10 | AT2G37870 | 5.4 | 4.6 | 2.3E-21 | 6.9 | 1.3E-25 | 5.6 | 5.2E-24 | 5.1 | 8.6E-23 | 1 | |
| D | S | – | C | Φ-1-like protein | RAFL03-02-F02 | AT5G64260 | 4.8 | 6.9 | 4.5E-26 | 5.6 | 6.9E-24 | 4.1 | 6.8E-20 | 4.1 | 9.1E-20 | 3 | |
| D | S | A | C | KIN2 | LEA class protein | RAFL04-17-B12 | AT5G15970 | 4.8 | 4.0 | 7.3E-20 | 5.8 | 2.4E-24 | 4.5 | 2.0E-21 | 5.1 | 5.2E-23 | 6 |
| – | – | – | – | Putative isocitrate lyase | RAFL06-07-C24 | AT3G21720 | 4.7 | 5.0 | 1.5E-22 | 5.1 | 2.2E-22 | 4.5 | 3.6E-21 | 3.4 | 3.3E-17 | 1 | |
| D | – | – | C | WD 40 repeat protein | RAFL05-19-N20 | AT1G24530 | 4.3 | 4.1 | 1.9E-19 | 4.5 | 7.5E-20 | 2.3 | 6.9E-11 | 6.0 | 7.6E-24 | 1 | |
| – | – | – | – | ORG2 | bHLH proteini | AT3G56970 | 4.2 | 2.0 | 4.7E-07 | 2.3 | 1.8E-06 | 7.6 | 1.6E-26 | 6.2 | 3.2E-24 | 0 | |
| – | – | – | – | Jasmonate inducible protein, putative | RAFL16-34-L18 | AT1G52100 | 4.2 | 3.5 | 3.8E-17 | 3.5 | 1.7E-16 | 5.1 | 1.4E-22 | 4.9 | 6.8E-22 | 3 | |
| D | – | – | – | Senescence-associated protein | RAFL02-09-F24 | AT5G66170 | 4.2 | 4.2 | 1.5E-19 | 4.1 | 5.6E-18 | 4.5 | 1.2E-20 | 3.6 | 3.5E-17 | 3 | |
| – | – | A | – | GBF2 | Transcription factor | RAFL08-16-I23 | AT4G01120 | 3.9 | 4.3 | 2.5E-20 | 4.2 | 1.3E-19 | 3.6 | 3.8E-18 | 3.0 | 8.0E-15 | 7 |
| D | S | A | – | SAG29 | Senescence-associated protein | RAFL05-19-F21 | AT5G13170 | 3.9 | 4.3 | 1.1E-20 | 3.8 | 7.1E-19 | 4.0 | 1.3E-19 | 3.6 | 5.0E-18 | 4 |
| – | – | – | – | Invertase-like protein | RAFL08-08-F02 | AT4G34860 | 3.6 | 3.0 | 3.2E-14 | 2.3 | 7.3E-10 | 4.3 | 4.4E-20 | 4.8 | 5.6E-20 | 2 | |
| – | – | A | – | Lipase class 3 family protein | RAFL05-18-O21 | AT2G30550 | 3.5 | 3.0 | 8.7E-15 | 3.1 | 4.2E-15 | 3.9 | 3.2E-19 | 4.0 | 7.0E-19 | 1 | |
| D | – | – | – | Dormancy/auxin-associated family protein | RAFL05-13-B18 | AT1G56220 | 3.5 | 4.0 | 1.3E-19 | 5.3 | 1.7E-23 | 2.7 | 1.7E-13 | 3.0 | 2.1E-15 | 2 | |
| D | – | A | – | Glycosyl hydrolase family 36 | RAFL05-19-C02 | AT3G57520 | 3.4 | 4.4 | 5.8E-21 | 3.9 | 6.7E-19 | 3.0 | 4.3E-15 | 2.5 | 9.0E-12 | 3 | |
| – | – | – | – | ROC1 | Cyclosporin A binding protein | RAFL05-21-A06 | AT4G38740 | 3.4 | 3.2 | 2.9E-16 | 2.9 | 7.1E-15 | 3.7 | 2.0E-18 | 3.8 | 6.4E-19 | 2 |
| – | – | – | – | ELIP2 | Early light-induced protein, putative | RAFL04-12-M20 | AT4G14690 | 3.4 | 3.5 | 2.3E-17 | 2.9 | 1.9E-14 | 3.9 | 1.8E-19 | 3.2 | 2.3E-16 | 3 |
| – | – | – | – | FLS1 | Flavonol synthase 1 | RAFL09-32-C09 | AT5G08640 | 3.3 | 2.7 | 4.6E-13 | 2.6 | 1.2E-11 | 4.0 | 1.9E-19 | 3.9 | 5.5E-19 | 2 |
| – | – | – | – | ORG3 | bHLH protein | AT3G56980 | 3.3 | 1.7 | 1.3E-04 | 2.6 | 6.4E-07 | 4.0 | 7.8E-19 | 6.4 | 4.2E-22 | 0 | |
| – | – | – | – | ENTH domain-containing protein | AT4G32285 | 3.2 | 3.8 | 2.2E-18 | 2.6 | 1.6E-11 | 4.6 | 2.8E-21 | 2.7 | 7.0E-13 | 0 | ||
| – | – | – | – | Glutathione S-transferase, putative | RAFL14-52-N20 | AT1G17190 | 3.2 | 2.2 | 1.5E-09 | 3.2 | 6.9E-14 | 3.1 | 1.5E-15 | 4.2 | 4.1E-19 | 0 | |
| – | – | – | – | Aldose reductase, putative | RAFL19-21-M03 | AT5G01670 | 3.1 | 2.1 | 9.0E-09 | 3.2 | 2.2E-14 | 5.4 | 5.4E-23 | 3.0 | 1.1E-14 | 1 | |
| – | – | – | – | LTP4 | Lipid transfer protein 4 | RAFL05-08-P24 | AT5G59310 | 3.1 | 3.6 | 7.5E-18 | 4.9 | 2.0E-20 | 2.3 | 1.4E-10 | 2.5 | 1.3E-11 | 1 |
| – | S | – | C | Lipid-associated protein family | RAFL14-89-N02 | AT2G22170 | 3.1 | 2.9 | 6.3E-15 | 3.3 | 6.9E-17 | 3.0 | 2.7E-15 | 3.1 | 7.6E-16 | 0 | |
a
Upregulated genes in untreated 35S-AREB1ΔQT transgenic plants (AREB1ΔQT/control). A complete data set is available at arraExpress (http://www.ebi.ac.uk/arrayex/query/entry) under accession number E-MEXP-397.
b
Data on inducibility were based on microarray analysis (Seki et al., 2002; K. Maruyama and K. Yamaguchi-Shinozaki, unpublished data). D, drought; S, high salinity; A, ABA; C, cold.
c
Description as given by The Institute for Genomic Research database.
d
RAFL, RIKEN Arabidopsis full-length cDNA.
e
AGI, Arabidopsis Genome Initiative.
f
Genes with median of fold change (untreated 35S-AREB1ΔQT plants/untreated control plants) of >3 are listed.
g
P values < 0.001 were studied.
h
Number of ABRE core sequences in 1000 bp of the sequence upstream of the gene.
i
bHLH, basic helix-loop-helix.