Figure 8.

RNA Gel Blot Analysis of PHF1 Responsiveness to Different Developmental and Nutritional Signals, and the Effect of phr1.

(A) PHF1 expression in response to developmental cues. Wild-type plants were grown for 4 weeks in soil or 2 weeks in complete medium for root isolation, and then organs were collected independently before RNA extraction: flowers (F), siliques (S), stems (St), cauline leaves (CL), nonsenescent rosette leaves (RL), senescent rosette leaves (SRL), and roots (R).

(B) PHF1 responsivenes to nutritional signals. RNA was extracted from total wild-type plants grown in solid medium for 5 d and then transferred for 5 d to medium containing (control [Ct]) or lacking Pi (−P), potassium (−K), sulfur (−S), iron (−Fe), nitrogen (−N), or sucrose (−Suc).

(C) PHR1 control of PHF1. RNA was extracted from wild-type, phf1-1, and phr1-1 plants grown for 8 d in complete (+P) or Pi-deficient (−P) medium. In all cases, RNA gel blots containing 15 μg of RNA per track were successively hybridized to the probes indicated.