Figure 5.

Representative images of immunocytochemistry with Hoescht (blue) and γH2AX (green) following proton treatment (14 MeV, 5 Gy) in U87 cells at (a) 1 hour and (b) 24 hours post-irradiation (20x magnification, scale bar: 50 µm). (c) Image quantification as γH2AX total fluorescence intensity per nucleus (fold to control: non-irradiated dishes at 1 hour post irradiation, Mean ± SEM). Three independent assays were performed with th imaging fields acquired per condition and a minimum of 100 cells quantified per field. Statistical differences were determined using a 2-way ANOVA followed by a Tukey’s test between treated and control conditions (* P < .05, ** P < .01, **** P < .0001).