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Proteinase K treatment of microsomes containing constructs 62(−G1, −G2), 67(−G1, −G2), and 68(−G1, −G2). mRNA was translated in the absence or presence of RMs, and samples were treated with proteinase K (PK) as indicated. The top panel is for a carboxy tail length of 72 residues, and the bottom panel for a carboxy tail length of 18 residues. Note that both the G1 and G2 glycosylation acceptor sites were removed in these constructs.
