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. 2026 May 10;14(9):e70911. doi: 10.14814/phy2.70911

FIGURE 5.

FIGURE 5

Mitochondrial morphology of SBG2‐(T8993G) hiPSC, in comparison to healthy control BJ‐hiPSC. Representative Phase contrast, Mitotracker Red, Nucblue, and Skeletonized images (a) are demonstrated. Different mitochondrial morphological parameters were determined and analyzed in comparison with the BJ‐hiPSC (control cell line) using MitoCellPhe to quantify the mitochondrial skeleton area (b), punctate count (c), rod count (d), network count (e), mean network branch count (f), mean rod length (g), mean network length (h), and mean lengths of all branches (i). All data are representative of five to seven analyzed images obtained from seven to nine independent dishes from three independent experiments. The bars represent minimum and maximum values, including all points, and each black dot represents a different data point. The gray bars represent the BJ‐control hiPSC, whereas the pink bars represent SBG2‐(T8993G)‐hiPSC. Scale bar = 100 μm.