Figure 3.

Primer extension by P1 DNA polymerase Y1 and P2 DNA polymerase Y1. Cy3-labelled-primer 2/template 2 (0.1 μM) was incubated with P1 DNA polymerase Y1 (200 nM) or P2 DNA polymerase Y1 (25 nM) in the presence of 50 μM each of dNTPs. The incubations were carried out in the presence of different combinations of dNTPs (lanes 3–6, both panels). The reaction mixtures were incubated for 30 min at 55 °C.