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. 2003 Feb 7;4(3):269–273. doi: 10.1038/sj.embor.embor765

Figure 6.

Figure 6

Incorporation of ribonucleotide triphosphates (rNTPs), 8-OH-dGTP and 2-OH-dATP by P1 DNA polymerase Y1 and the F12A mutant. Cy3-labelled-primer 2/template 2 (0.1 μM) was incubated with 200 nM of P1 DNA polymerase Y1 (A) or 400 nM of the F12A mutant enzyme (B) in the presence of rNTPs for 10 min at 55 °C. Manganese ions (1.5 mM) were also added to the reaction mixture containing 500 μM rNTP in the lanes labelled 'Mn2+'. The template/primer solution (0.1 μM) was incubated with the F12A enzyme (400 nM) in the presence of 50 μM 8-OH-dGTP (C) or 50 μM 2-OH-dATP (D) for 30 min at 55 °C.