Figure 1.
Expression of yellow fluorescent protein (YFP) fusion constructs in human embryonic kidney (HEK)-293 cells. Cells were transfected with complementary DNAs encoding either YFP or YFP-tagged protein-tyrosine phosphatase 1B (PTP1B) constructs. Expression of YFP in HEK-293 cells results in a fluorescent signal distributed uniformly throughout the cell (A). Expression of wild-type YFP–PTP1B (B) or YFP–PTP1B–D181A (C) results in an intracellular and perinuclear distribution of the fluorescence.