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. 2003 Feb 21;4(3):296–300. doi: 10.1038/sj.embor.embor771

Figure 2.

Figure 2

Synteny of the his/trp region and sequence similarity of PriA and HisA. (A) Organization of the his/trp cluster of Streptomyces coelicolor (Bentley et al., 2002) and Mycobacterium tuberculosis (Cole et al., 1998). The hisA homologues of S. coelicolor (SCO2050) and M. tuberculosis (Rv1603) are marked with asterisks. Genes involved in histidine (his; black) and tryptophan (trp; grey) biosynthesis are shown. (B) Sequence alignment of the HisA homologues of S. coelicolor (SC–PriA) and Thermotoga maritima (TM–HisA), which show 25% identity. Data from T. maritima (Jürgens et al., 2000; Hennsax et al., 2002) were used to identify the catalytically important residues in SCO2050 (Asp 11, Asp 130 and Thr 166 (asterisks)). The region of SCO2050 replaced in WH101 (this study) lies between Ala 126 and Gly 210 (indicated by arrows).