Table 2.
Plasmid | Strain | ||
W3110 trpC(Fdel) | Hfr G6 | ||
pGEX-4T-1 | − | − | |
pGEX–PriASc | + | + | |
pGEX–PriAMt | + | + | |
pGEX–HisAEc | − | + | |
pGEX–TrpFEc | + | − |
Complementation detected (+) or not detected (−) by expression of the insert from the lacI-dependent promoter induced with 10 μM isopropylthiogalactoside.