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. 2003 Feb 21;4(3):296–300. doi: 10.1038/sj.embor.embor771

Table 2.

Complementation of Escherichia coli strains W3110 trpC (Fdel) and Hfr G6

Plasmid Strain    
  W3110 trpC(Fdel) Hfr G6  
pGEX-4T-1  
pGEX–PriASc + +  
pGEX–PriAMt + +  
pGEX–HisAEc +  
pGEX–TrpFEc +  

Complementation detected (+) or not detected (−) by expression of the insert from the lacI-dependent promoter induced with 10 μM isopropylthiogalactoside.