FIG. 4.

(A) Effects of a potent inhibitor of herpesvirus TK, 5′-ET (43), added at 10, 20, or 50 μM on the anti-KSHV activity of N-MCT (1 μM) and CDV (10 μM) in PMA-stimulated (PMA+) BCBL-1 cells. Shown are the levels of KSHV DNA evaluated by ORF65 PCR and mitochondrial DNA in the Hirt LMW DNA. (B) The effects of 5′-ET (50 μM) on anti-KSHV activity of N-MCT used at 1, 3, or 10 μM in PMA-stimulated BCBL-1. Shown are the amounts of virion-associated (supernatants) and cell-associated KSHV DNA (LMW) as determined by ORF65 PCR along with the levels of control mitochondrial DNA. (C) The levels of phosphorylated metabolites of N-MCT added at 1, 3, or 10 μM in the absence (top) or presence (bottom) of 50 μM 5′-ET in PMA-stimulated BCBL-1 cells.