FIG. 2.
Z factor determination. Eighty-eight wells of a 96-well plate were seeded with 24ST1NLESG cells (105/well) either in medium alone or in medium containing an inducer in a final volume of 20 μl. The remaining eight wells contained serial dilutions of alkaline phosphatase protein as a positive control for the assay. The results from 88 samples per plate were compiled and used to determine the Z factor. Both uninduced (gray circles) and induced (black diamonds) cells were assayed for SEAP activity after 48 h. Cells were treated with 50 ng/ml TNF-α (A), 1 mM VPA (B), and 100 ng/ml phorbol 12-myristate 13-acetate (PMA) (C). RLU, relative light units.