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. 2005 Dec;49(12):4876–4883. doi: 10.1128/AAC.49.12.4876-4883.2005

FIG. 2.

FIG. 2.

Overexpression and purification of the recombinant OM proteins CarO and Omp25 of A. baumannii ATCC 19606. (A) SDS-PAGE (12.5%) with Coomassie blue staining. Lane 1, membrane fraction before overexpression; lanes 2 and 3, membrane fraction containing overproduced CarO heated for 10 min at 100°C or without heating, respectively; lanes 4 and 5, purified CarO with or without heating, respectively; lanes 6 and 7, membrane fraction containing overproduced Omp25 with or without heating, respectively; lanes 8 and 9, purified Omp25 with or without heating, respectively. The molecular mass and position of each of the different standards (lane M) are shown on the left. (B) Immunoblot analysis of the same samples with antibodies directed against the His-tagged CarO and Omp25 OM proteins.