FIG. 4.
Single-channel recordings of native and recombinant A. baumannii OM proteins. Proteins (5 × 10−9 M) were reconstituted into DPhPC planar bilayers in 1 M KCl-10 mM HEPES, pH 7.4. (A) Triton X-100-solubilized CarO and Omp25. The recordings show different current levels for an applied potential of 120 mV. (B) Left panel, selected recording showing the single-channel activity of CarO at an applied voltage of 80 mV. O, open state at different current levels; C, closed state. Right panel, histogram associated with the recording showing that the different current levels correspond to similar conductance values: 105 ± 15 pS.