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. 2026 May 6;48(1):2666452. doi: 10.1080/0886022X.2026.2666452

Figure 4.

Multi-panel figure displaying Western blot analyses and bar graphs of p-EGFR and EGFR levels across various siRNA treatments and MTX concentrations. The figure includes six panels (A-F) showing Western blot results and bar graphs. Panel A compares p-EGFR, EGFR, and GAPDH levels for Scramble and USP11 siRNA with or without UA treatment. Panel B features bar charts illustrating p-EGFR/EGFR and EGFR/GAPDH abundance differences between siRNA conditions. Panel C examines p-EGFR response under varying MTX concentrations (0-10 µM), showing decreasing intensity with higher MTX. Panel D contains corresponding bar graphs for these results. Panel E compares p-EGFR and EGFR levels between pcDNA 3.0 NC and USP11 treatments. Finally, Panel F presents bar graphs quantifying p-EGFR/EGFR ratios, highlighting significant differences between groups.

USP11 activates EGFR signaling pathway in the UA-stimulated NRK49F. Western blot analysis of p-EGFR and EGFR protein expressions in each group were conducted, with normalization to GAPDH (A, C, E). Quantitative analysis of p-EGFR and EGFR protein levels in each group (B, D, F). Data were expressed as mean ± SD (n = 4 for each group). N.S.: no significant difference. ***p < 0.001, ****p < 0.0001.