FIG. 4.

Determination of ATPase activity and compound inhibition for HPV16, HPV18, and CRPV E1 proteins. Scintillation proximity ATPase assays were performed using in vitro-translated E1 helicase domains immobilized via the anti-FLAG antibody. Raw cpm are shown for negative controls (in vitro translation reactions programmed with empty pTM1 vector alone; white bars), and E1 reactions in the absence of inhibitor (black) or in the presence of 80 μM compound 5 (dark gray) or 9 (light gray). Error bars correspond to standard deviations for quadruplicate values. Values for percent inhibition were calculated by comparing blank-subtracted reactions in the presence and absence of inhibitor and are given above the corresponding gray bars.