TABLE 1.
Primers used for detection of erythromycin resistance determinants and genetic carrying elements in Streptococcus agalactiae strains
| Primer group or target gene | Forward and reverse primers (5′-3′) | Amplification size (bp) | Nucleotide positions for genetic element analyzedb
|
|||
|---|---|---|---|---|---|---|
| Tn1207.3 (AY657002) | Mega (AF274302) | Tn916 (U09422) | Tn2009 (AF376746) | |||
| erm(B)a | ATTGGAACAGGTAAAGGGC | 442 | ||||
| GAACATCTGTGGTATGGCG | ||||||
| erm(TR) | AACTTGTGGAAATGAGTCAACGG | 375 | ||||
| CAGAATCTACATTAGGCTTAGGG | ||||||
| Primers for analysis of Tn1207.3 and mega | ||||||
| mef(A), mef(E) | ATGGAAAAATACAACAATTGG | 1,218 | 3271-3291 | 1125-1145 | 1372-1392 | |
| TTATTTTAAATCTAATTTTCTAA(C/T)C | 4464-4488 | 2318-2342 | 2565-2589 | |||
| matA, mela | TGCCTATATTCCCCAGTT | 708 | 4808-4825 | 2662-2679 | 2909-2926 | |
| TAATTTCCGCACCGACTA | 5497-5514 | 3351-3368 | 3598-3615 | |||
| orf8, orf5 | ATGACCGCCGATAAGTTTACTAC | 291 | 6870-6892 | 5074-5094 | 5319-5339 | |
| GACCCAGCAAATCTTCCAAAAAC | 7138-7160 | 5342-5364 | 5586-5608 | |||
| orf9 | ATGACCGCCGATAAGTTTACTAC | 699 | 6870-6892 | |||
| GTATGGGCCATTCCAAAACTAAC | 7546-7568 | |||||
| orf56 | ATATGCGAGGGTATCTGATAGTC | 495 | 49036-49058 | |||
| GGCTCAATGACATAATTCTCACC | 49508-49530 | |||||
| Primers for analysis of mega and Tn2009 | ||||||
| orf7 | TCAATTTATCAATAAATGAAATGAG | 205 | 516-540 | 781-805 | ||
| ATACTTCCAATTCGATGCCAG | 700-720 | 964-984 | ||||
| intTn | GGTCTTCGTATTTCAGAGTTTGG | 473 | 17307-17329 | |||
| GTTGCATGTGCGTAATAGTTCAG | 17766-17788 | |||||
| orf24 | TCAAAAGGTCGTTCCCCACTC | 344 | 260-280 | |||
| ACGAGTGCCGATTTTGTAGCC | 582-602 | |||||
| orf5-orf6-tet(M) | ORF5-F: TAATACAAGTACATGACTCACC | 585 | 5316-5337 | 5561-5582 | ||
| TetM-R: TCCTAAATATTGTGCGAACATC | 6123-6144 | |||||
a
From reference 1.
b
GenBank accession numbers are given in parentheses.