FIG. 4.

High relative fitness of SIVMne170 in rapid turnover systems. Cultures were inoculated with SIVMneCL8- and SIVMne170-infected cells mixed in different ratios. The virus was transferred from infected cultures to uninfected cells by transferring 1/10 of cells (A) or incubating cell-free virus with DC-SIGN-expressing Raji B cells (B) and mixing these cells with uninfected CEMx174 cells. The proportion of SIVMneCL8 RNA in culture medium was determined before the first transfer and after each consecutive transfer. Each line represents an independent competition and shows the reduction in proportion of SIVMneCL8 after each transfer, indicating higher fitness of SIVMne170 in the rapid turnover assays.