FIG. 2.

Growth of TNF-α-expressing recombinant RV in NA cells. NA cells were infected with SPBN-TNF-α(−), SPBN-TNF-α(MEM), or SPBN-TNF-α(+) at an MOI of 5 (A) or 0.01 (B) and incubated at 34°C. At the indicated times p.i., viruses were harvested and titrated. All titrations were carried out in quadruplicate, and titers are expressed as mean values ± SEM. The statistical significance among titers produced by the different recombinant RVs was determined using one-way ANOVA with the Newman-Keuls multiple comparison test.