FIG. 6.

Detection of RV-infected neurons in the brain. The images show the presence of rabies virus RNP in neurons of the cortexes (A, C, E) and the CA1-to-CA3 region of the hippocampal formations (B, D, F) of TNF-α KO mice on day 10 after infection with SPBN-TNF-α(+), SPBN-TNF-α(MEM), or SPBN-TNF-α(−). Bright-field microscopy of immunostained brain sections is shown. DG, dentate gyrus; CA, CA region of hippocampus. Quantitation of rabies virus-infected neurons (RNP) in the cerebral cortexes (G) and the CA1-to-CA3 region of the hippocampal formations (H) of TNF-α KO mice infected with SPBN-TNF-α(+), SPBN-TNF-α(MEM), or SPBN-TNF-α(−) was done at 10 days p.i. Data are expressed as mean values ± SEM; asterisks indicate significant differences (***, P < 0.001) between experimental groups, as calculated by ANOVA.